Periostin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54359
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54359-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54359-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the POSTN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with POSTN antibody; Blue=isotype control, Green= POSTN antibody.
2 / 4
IHC testing of FFPE human breast tissue with POSTN antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human A375 cells with POSTN antibody (green) and DAPI nuclear stain (blue).
4 / 4
Western blot testing of 1) human breast and 2) rat lung lysate with POSTN antibody. Predicted molecular weight ~93 kDa.
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Flow cytometry testing of fixed and permeabilized human HeLa cells with POSTN antibody; Blue=isotype control, Green= POSTN antibody.
IHC testing of FFPE human breast tissue with POSTN antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human A375 cells with POSTN antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human breast and 2) rat lung lysate with POSTN antibody. Predicted molecular weight ~93 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the POSTN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
POSTN binds to heparin. It induces cell attachment and spreading and plays a role in cell adhesion and may play a role in extracellular matrix mineralization.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 687-716 from the human protein was used as the immunogen for the POSTN antibody.
Limitation:
This POSTN antibody is available for research use only.
Localization:
Cytoplasm, extracellular space
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
Q15063