HSPA5 Antibody / GRP78 / BiP

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NSJ Bioreagents
Product Code: NSJ-F54813
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54813-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54813-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSPA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HeLa, 2) A2058 and 3) NCI-H460 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
2 / 4
Western blot testing of zebrafish brain lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
3 / 4
Immunofluorescent staining of human HeLa cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human lung carcinoma tissue with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HeLa, 2) A2058 and 3) NCI-H460 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Western blot testing of zebrafish brain lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Immunofluorescent staining of human HeLa cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human lung carcinoma tissue with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSPA5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 261-289 from the human protein was used as the immunogen for the HSPA5 antibody.
Limitation:
This HSPA5 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Zebrafish
Uniprot #:
P11021