CKI-gamma 3 Antibody / Csnk1g3

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NSJ Bioreagents
Product Code: NSJ-F54677
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54677-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54677-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CKI-gamma 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE mouse cerebellum tissue with CKI-gamma 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE mouse liver tissue with CKI-gamma 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of mouse 1) kidney and 2) liver lysate with CKI-gamma 3 antibody. Predicted molecular weight ~49 kDa.
4 / 4
Flow cytometry testing of mouse NIH 3T3 cells with CKI-gamma 3 antibody; Blue=isotype control, Green= CKI-gamma 3 antibody.
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IHC testing of FFPE mouse cerebellum tissue with CKI-gamma 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse liver tissue with CKI-gamma 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse 1) kidney and 2) liver lysate with CKI-gamma 3 antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of mouse NIH 3T3 cells with CKI-gamma 3 antibody; Blue=isotype control, Green= CKI-gamma 3 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CKI-gamma 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 333-361 from the mouse protein was used as the immunogen for the CKI-gamma 3 antibody.
Limitation:
This CKI-gamma 3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Mouse
Uniprot #:
Q8C4X2