Tak1 Antibody / Map3k7

NSJ Bioreagents
Product Code: NSJ-F54678
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54678-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54678-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Map3k7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE mouse stomach tissue with Map3k7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of mouse heart tissue lysate with Map3k7 antibody. Predicted molecular weight: 64-69 kDa, routinely observed at 78-82 kDa.
3 / 3
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Map3k7 antibody. Predicted molecular weight: 64-69 kDa, routinely observed at 78-82 kDa.

IHC testing of FFPE mouse stomach tissue with Map3k7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse heart tissue lysate with Map3k7 antibody. Predicted molecular weight: 64-69 kDa, routinely observed at 78-82 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Map3k7 antibody. Predicted molecular weight: 64-69 kDa, routinely observed at 78-82 kDa.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Map3k7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 131-160 from the mouse protein was used as the immunogen for the Map3k7 antibody.
Limitation:
This Map3k7 antibody is available for research use only.
Localization:
Cytoplasmic, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Mouse
Uniprot #:
Q62073