hsp90 alpha Antibody / hsp90a.1

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NSJ Bioreagents
Product Code: NSJ-F54476
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54476-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54476-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Zebrafish
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the hsp90a.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of zebrafish 1) muscle and 2) whole lysate with hsp90a.1 antibody. Predicted molecular weight ~83 kDa.
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Western blot testing of zebrafish heart lysate with hsp90a.1 antibody. Predicted molecular weight ~83 kDa.
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Western blot testing of zebrafish ZF4 cell lysate with hsp90a.1 antibody. Predicted molecular weight ~83 kDa.
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IHC testing of FFPE human skeletal muscle tissue with hsp90a.1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of zebrafish 1) muscle and 2) whole lysate with hsp90a.1 antibody. Predicted molecular weight ~83 kDa.
Western blot testing of zebrafish heart lysate with hsp90a.1 antibody. Predicted molecular weight ~83 kDa.
Western blot testing of zebrafish ZF4 cell lysate with hsp90a.1 antibody. Predicted molecular weight ~83 kDa.
IHC testing of FFPE human skeletal muscle tissue with hsp90a.1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the hsp90a.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). Plays a key role in slow and fast muscle development in the embryo. Plays a role in myosin expression and assembly.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 229-263 from the zebrafish protein was used as the immunogen for the hsp90a.1 antibody.
Limitation:
This hsp90a.1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Zebrafish
Uniprot #:
Q90474