ak2 Antibody (Zebrafish)

NSJ Bioreagents
Product Code: NSJ-F54506
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54506-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54506-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Zebrafish
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the zebrafish ak2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE zebrafish body tissue with zebrafish ak2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human heart tissue with zebrafish ak2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of 1) whole zebrafish, 2) zebrafish ZF4, 3) human heart and 4) mouse liver lysate with zebrafish ak2 antibody. Predicted molecular weight ~26 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized zebrafish ZF4 cells with zebrafish ak2 antibody; Blue=isotype control, Green= ak2 antibody.

IHC testing of FFPE zebrafish body tissue with zebrafish ak2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human heart tissue with zebrafish ak2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) whole zebrafish, 2) zebrafish ZF4, 3) human heart and 4) mouse liver lysate with zebrafish ak2 antibody. Predicted molecular weight ~26 kDa.
Flow cytometry testing of fixed and permeabilized zebrafish ZF4 cells with zebrafish ak2 antibody; Blue=isotype control, Green= ak2 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the zebrafish ak2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the zebrafish ak2 antibody.
Limitation:
This Zebrafish ak2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Zebrafish, Human, Mouse
Uniprot #:
Q1L8L9