Claudin 5 Antibody / CLDN5

NSJ Bioreagents
Product Code: NSJ-RQ5715
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5715-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the CLDN5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
IHC staining of FFPE human rectal cancer with CLDN5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 2
Immunofluorescent staining of FFPE human intestinal cancer with CLDN5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

IHC staining of FFPE human rectal cancer with CLDN5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer with CLDN5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the CLDN5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KYSAPRRPTATGDYDKKNYV from the human protein were used as the immunogen for the CLDN5 antibody.
Limitation:
This CLDN5 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O00501