c-Fos Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5724
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5724-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the c-Fos antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human SiHa cells with c-Fos antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= c-Fos antibody.
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) SH-SY5Y and 4) A549 cell lysate with c-Fos antibody. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated).
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Flow cytometry testing of human SiHa cells with c-Fos antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= c-Fos antibody.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) SH-SY5Y and 4) A549 cell lysate with c-Fos antibody. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the c-Fos antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins(FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1(AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al.(1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N45-D293) was used as the immunogen for the c-Fos antibody.
Limitation:
This c-Fos antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P01100