DBF4 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ5689
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5689-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the DBF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human SH-SY5Y cells with DBF4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human ThP-1 cells with DBF4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DBF4 antibody.
3 / 3
Western blot testing of human 1) Jurkat and 2) SW620 lysate with DBF4 antibody. Predicted molecular weight: ~74 kDa.
❮❯

Immunofluorescent staining of FFPE human SH-SY5Y cells with DBF4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human ThP-1 cells with DBF4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DBF4 antibody.
Western blot testing of human 1) Jurkat and 2) SW620 lysate with DBF4 antibody. Predicted molecular weight: ~74 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DBF4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y295-S665) was used as the immunogen for the DBF4 antibody.
Limitation:
This DBF4 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UBU7