CD168 Antibody / HMMR

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NSJ Bioreagents
Product Code: NSJ-RQ5628
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5628-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the HMMR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with HMMR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with HMMR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
Flow cytometry testing of human U937 cells with HMMR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HMMR antibody.
4 / 4
Western blot testing of human 1) HeLa, 2) Jurkat, 3) SW620, 4) T-47D and 5) Raji lysate with HMMR antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
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Immunofluorescent staining of FFPE human U-2 OS cells with HMMR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with HMMR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with HMMR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HMMR antibody.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) SW620, 4) T-47D and 5) Raji lysate with HMMR antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HMMR antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Hyaluronan-mediated motility receptor (HMMR), also known as RHAMM (Receptor for Hyaluronan Mediated Motility) is a protein which in humans is encoded by the HMMR gene. It is mapped to 5q34. The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K5-D686) was used as the immunogen for the HMMR antibody.
Limitation:
This HMMR antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O75330