OAS3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5634
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5634-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the OAS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HeLa cells with OAS3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human SiHa cells with OAS3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OAS3 antibody.
3 / 3
Western blot testing of human 1) HeLa, 2) PC-3, 3) HepG2 and 4) A549 lysate with OAS3 antibody. Predicted molecular weight ~121 kDa.
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Immunofluorescent staining of FFPE human HeLa cells with OAS3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with OAS3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OAS3 antibody.
Western blot testing of human 1) HeLa, 2) PC-3, 3) HepG2 and 4) A549 lysate with OAS3 antibody. Predicted molecular weight ~121 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the OAS3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
2'-5'-oligoadenylate synthetase 3 is an enzyme that in humans is encoded by the OAS3 gene. It is mapped to 12q24.13. This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N990-V1087) was used as the immunogen for the OAS3 antibody.
Limitation:
This OAS3 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9Y6K5