Desmoglein 2 Antibody / DSG2

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NSJ Bioreagents
Product Code: NSJ-RQ5646
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5646-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Desmoglein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human prostate cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human appendicitis tissue with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
Immunofluorescent staining of FFPE human MCF7 cells with Desmoglein 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 8
Western blot testing of human 1) placenta, 2) K562, 3) HeLa, 4) U-2 OS, 5) HepG2 and 6) PC-3 lysate with Desmoglein 2 antibody. Expected molecular weight: 122-160 kDa depending on glycosylation level.
6 / 8
Flow cytometry testing of human U-87 MG cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
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Flow cytometry testing of mouse HEPA1-6 cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
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Flow cytometry testing of rat RH35 cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
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IHC staining of FFPE human prostate cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendicitis tissue with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with Desmoglein 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) K562, 3) HeLa, 4) U-2 OS, 5) HepG2 and 6) PC-3 lysate with Desmoglein 2 antibody. Expected molecular weight: 122-160 kDa depending on glycosylation level.
Flow cytometry testing of human U-87 MG cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
Flow cytometry testing of rat RH35 cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Desmoglein 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids L24-E1020) was used as the immunogen for the Desmoglein 2 antibody.
Limitation:
This Desmoglein 2 antibody is available for research use only.
Localization:
Cytoplasm, membrane (cell junctions)
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q14126