Neurofibromin Antibody / NF1

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NSJ Bioreagents
Product Code: NSJ-RQ5649
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5649-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Neurofibromin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with Neurofibromin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE human breast cancer with Neurofibromin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human rectal cancer with Neurofibromin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Western blot testing of human HeLa cell lysate with Neurofibromin antibody. Predicted molecular weight ~319 kDa.
5 / 6
Flow cytometry testing of human HeLa cells with Neurofibromin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofibromin antibody.
6 / 6
Flow cytometry testing of human A549 cells with Neurofibromin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofibromin antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Neurofibromin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer with Neurofibromin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Neurofibromin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human HeLa cell lysate with Neurofibromin antibody. Predicted molecular weight ~319 kDa.
Flow cytometry testing of human HeLa cells with Neurofibromin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofibromin antibody.
Flow cytometry testing of human A549 cells with Neurofibromin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofibromin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Neurofibromin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Neurofibromin 1 (NF1) is a gene in humans that is located on chromosome 17. This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R160-Q270) was used as the immunogen for the Neurofibromin antibody.
Limitation:
This Neurofibromin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P21359