ATG9A Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5704
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5704-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the ATG9A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A549 cells with ATG9A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human U-87 MG cells with ATG9A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG9A antibody.
3 / 3
Western blot testing of human 1) HepG2, 2) A549, 3) A375, 4) MCF-7, 5) K562 and 6) mouse testis lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
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Immunofluorescent staining of FFPE human A549 cells with ATG9A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-87 MG cells with ATG9A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG9A antibody.
Western blot testing of human 1) HepG2, 2) A549, 3) A375, 4) MCF-7, 5) K562 and 6) mouse testis lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ATG9A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Autophagy-related protein 9A is a protein that in humans is encoded by the ATG9A gene. ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D812) was used as the immunogen for the ATG9A antibody.
Limitation:
This ATG9A antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q7Z3C6