CISD1 Antibody / MitoNEET

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NSJ Bioreagents
Product Code: NSJ-RQ5710
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5710-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MitoNEET antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human rectal cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human SW620, 2) monkey heart, 3) rat heart, 4) rat kidney and 5) mouse heart lysate with MitoNEET antibody. Expected molecular weight: 12-15 kDa.
6 / 6
Flow cytometry testing of human PC-3 cells with MitoNEET antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MitoNEET antibody.
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IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SW620, 2) monkey heart, 3) rat heart, 4) rat kidney and 5) mouse heart lysate with MitoNEET antibody. Expected molecular weight: 12-15 kDa.
Flow cytometry testing of human PC-3 cells with MitoNEET antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MitoNEET antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MitoNEET antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CISD1, also called MitoNEET, is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity. This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y28-E93) was used as the immunogen for the MitoNEET antibody.
Limitation:
This MitoNEET antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NZ45