DDX6 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5718
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5718-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DDX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A549 cells with DDX6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human breast cancer with DDX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) placenta, 2) HeLa, 3) HepG2, 4) A431, 5) Raji, 6) ThP-1, 7) rat PC-12, 8) mouse NIH 3T3 and 9) mouse HEPA1-6 lysate with DDX6 antibody. Predicted molecular weight ~53 kDa.
4 / 4
Flow cytometry testing of human HL-60 cells with DDX6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDX6 antibody.
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Immunofluorescent staining of FFPE human A549 cells with DDX6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer with DDX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) HeLa, 3) HepG2, 4) A431, 5) Raji, 6) ThP-1, 7) rat PC-12, 8) mouse NIH 3T3 and 9) mouse HEPA1-6 lysate with DDX6 antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human HL-60 cells with DDX6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDX6 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DDX6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
DDX6 (DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp(DEAD) box motif(Seto et al., 1995). Tunnacliffe et al.(1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites(STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al.(2005) found that RCK, EDC3(YJDC), and HEDLS(RCD8) coimmunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q56-P483) was used as the immunogen for the DDX6 antibody.
Limitation:
This DDX6 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P26196