Folliculin Antibody / FLCN

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NSJ Bioreagents
Product Code: NSJ-RQ5662
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5662-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Folliculin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 11
IHC staining of FFPE human bladder cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human bladder cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human rectal cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human rectal cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human liver cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human appendicitis tissue with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human skin cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
Immunofluorescent staining of FFPE human U-2 OS cells with Folliculin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 11
Western blot testing of human 1) A431, 2) SK-OV-3, 3) HepG2, 4) rat kidney and 5) mouse kidney lysate with Folliculin antibody. Expected molecular weight: 64-70 kDa.
10 / 11
Flow cytometry testing of human U937 cells with Folliculin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Folliculin antibody.
11 / 11
Flow cytometry testing of human Caco-2 cells with Folliculin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Folliculin antibody.
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IHC staining of FFPE human bladder cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendicitis tissue with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin cancer with Folliculin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with Folliculin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A431, 2) SK-OV-3, 3) HepG2, 4) rat kidney and 5) mouse kidney lysate with Folliculin antibody. Expected molecular weight: 64-70 kDa.
Flow cytometry testing of human U937 cells with Folliculin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Folliculin antibody.
Flow cytometry testing of human Caco-2 cells with Folliculin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Folliculin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Folliculin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Folliculin also known as FLCN, Birt-Hogg-Dube syndrome protein or FLCN_HUMAN is a protein that in humans is associated with Birt-Hogg-Dube syndrome and hereditary spontaneous pneumothorax. It is encoded by the Folliculin (FLCN) gene (alias BHD, FLCL) that acts as a tumor suppressor gene. It is mapped to 17p11.2. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids C8-K551) was used as the immunogen for the Folliculin antibody.
Limitation:
This Folliculin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8NFG4