Alpha Enolase Antibody / ENO1 / Enolase 1

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NSJ Bioreagents
Product Code: NSJ-RQ5668
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5668-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Enolase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human A431 cells with Enolase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC staining of FFPE human liver cancer with Enolase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE mouse testis with Enolase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat testis with Enolase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Western blot testing of human 1) HeLa, 2) HepG2, 3) SH-SY5Y, 4) U-87 MG, 5) HEK293, 6) Caco-2, 7) monkey kidney and 8) monkey liver lysate with Enolase 1 antibody. Predicted molecular weight ~47 kDa.
6 / 7
Western blot testing of rat 1) brain, 2) heart, 3) kidney, 4) liver, and mouse 5) brain, 6) kidney, 7) liver and 8) RAW264.7 lysate with Enolase 1 antibody. Predicted molecular weight ~47 kDa.
7 / 7
Flow cytometry testing of human HL-60 cells with Enolase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Enolase 1 antibody.
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Immunofluorescent staining of FFPE human A431 cells with Enolase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer with Enolase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with Enolase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with Enolase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HepG2, 3) SH-SY5Y, 4) U-87 MG, 5) HEK293, 6) Caco-2, 7) monkey kidney and 8) monkey liver lysate with Enolase 1 antibody. Predicted molecular weight ~47 kDa.
Western blot testing of rat 1) brain, 2) heart, 3) kidney, 4) liver, and mouse 5) brain, 6) kidney, 7) liver and 8) RAW264.7 lysate with Enolase 1 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human HL-60 cells with Enolase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Enolase 1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Enolase 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Enolase 1 (ENO1) is a glycolytic enzyme expressed in most tissues. It is mapped to 1p36.23. This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DDPSRYISPDQLADLYKSFIKD from the human protein were used as the immunogen for the Enolase 1 antibody.
Limitation:
This Enolase 1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P06733