DLL1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5690
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5690-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DLL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A549 cells with DLL1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human placenta with DLL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Flow cytometry testing of human ThP-1 cells with DLL1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DLL1 antibody.
4 / 4
Western blot testing of 1) human HeLa, 2) monkey COS-7 and human 3) Jurkat, 4) Raji, 5) SW620, 6) HepG2, 7) U-2 OS, 8) rat heart, 9) rat C6, 10) mouse heart, 11) mouse Neuro-2a, 12) mouse RAW264.7 lysate with DLL1 antibody. Predicted molecular weight ~78 kDa.
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Immunofluorescent staining of FFPE human A549 cells with DLL1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placenta with DLL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human ThP-1 cells with DLL1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DLL1 antibody.
Western blot testing of 1) human HeLa, 2) monkey COS-7 and human 3) Jurkat, 4) Raji, 5) SW620, 6) HepG2, 7) U-2 OS, 8) rat heart, 9) rat C6, 10) mouse heart, 11) mouse Neuro-2a, 12) mouse RAW264.7 lysate with DLL1 antibody. Predicted molecular weight ~78 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DLL1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. This gene is mapped to chromosome 6q27.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q18-K239) was used as the immunogen for the DLL1 antibody.
Limitation:
This DLL1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
O00548