Glutaminase Antibody / GLS

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NSJ Bioreagents
Product Code: NSJ-RQ5691
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5691-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GLS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with GLS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE human stomach cancer with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE rat brain with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Western blot testing of 1) human placenta, 2) human U-87 MG, 3) human HeLa, 4) monkey kidney, 5) rat brain, 6) rat kidney, 7) rat heart, 8) rat skeletal muscle, 9) mouse brain, 10) mouse kidney, 11) mouse heart, 12) mouse skeletal muscle and 13) mouse Neuro-2a antibody. Predicted molecular weigh: 65-73 kDa.
5 / 6
Flow cytometry testing of human 293T cells with GLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLS antibody.
6 / 6
Flow cytometry testing of human SiHa cells with GLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLS antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with GLS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human stomach cancer with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human placenta, 2) human U-87 MG, 3) human HeLa, 4) monkey kidney, 5) rat brain, 6) rat kidney, 7) rat heart, 8) rat skeletal muscle, 9) mouse brain, 10) mouse kidney, 11) mouse heart, 12) mouse skeletal muscle and 13) mouse Neuro-2a antibody. Predicted molecular weigh: 65-73 kDa.
Flow cytometry testing of human 293T cells with GLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLS antibody.
Flow cytometry testing of human SiHa cells with GLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLS antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GLS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K396-N654) was used as the immunogen for the GLS antibody.
Limitation:
This GLS antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
O94925