LRPPRC Antibody / GP130

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NSJ Bioreagents
Product Code: NSJ-RQ5633
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5633-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LRPPRC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human HeLa cells with LRPPRC antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC staining of FFPE human rectal cancer with LRPPRC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE mouse intestine with LRPPRC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat intestine with LRPPRC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Western blot testing of human 1) HEK293, 2) HeLa, 3) Jurkat and 4) Caco-2, 5) monkey kindey, 6) monkey heart, 7) rat heart, 8) rat skeletal muscle, 9) rat kidney, 10) rat liver and 11) mouse heart lysate with LRPPRC antibody. Expected molecular weight: 150-160 kDa.
6 / 7
Flow cytometry testing of human A431 cells with LRPPRC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LRPPRC antibody.
7 / 7
Flow cytometry testing of rat C6 cells with LRPPRC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LRPPRC antibody.
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Immunofluorescent staining of FFPE human HeLa cells with LRPPRC antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer with LRPPRC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with LRPPRC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with LRPPRC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEK293, 2) HeLa, 3) Jurkat and 4) Caco-2, 5) monkey kindey, 6) monkey heart, 7) rat heart, 8) rat skeletal muscle, 9) rat kidney, 10) rat liver and 11) mouse heart lysate with LRPPRC antibody. Expected molecular weight: 150-160 kDa.
Flow cytometry testing of human A431 cells with LRPPRC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LRPPRC antibody.
Flow cytometry testing of rat C6 cells with LRPPRC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LRPPRC antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LRPPRC antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Leucine-rich PPR motif-containing protein, mitochondrial is a protein that in humans is encoded by the LRPPRC gene. It is mapped to 2p21. This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A1281-S1394) was used as the immunogen for the LRPPRC antibody.
Limitation:
This LRPPRC antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P42704