LIPC Antibody / Hepatic Lipase

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NSJ Bioreagents
Product Code: NSJ-RQ5666
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5666-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Hepatic Lipase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human HepG2 cells with Hepatic Lipase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human liver cancer with Hepatic Lipase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE rat liver with Hepatic Lipase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Western blot testing of rat liver lysate with Hepatic Lipase antibody. Predicted molecular weight ~56 kDa.
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Immunofluorescent staining of FFPE human HepG2 cells with Hepatic Lipase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer with Hepatic Lipase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with Hepatic Lipase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of rat liver lysate with Hepatic Lipase antibody. Predicted molecular weight ~56 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Hepatic Lipase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Hepatic lipase (HL), also called hepatic triglyceride lipase (HTGL) or LIPC (for lipase, hepatic), is a form of lipase, catalyzing the hydrolysis of triacylglyceride. It is mapped to 8q24. LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant rat protein (amino acids E39-D494) was used as the immunogen for the Hepatic Lipase antibody.
Limitation:
This Hepatic Lipase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P07867