C5a-r Antibody / Complement C5A Receptor

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NSJ Bioreagents
Product Code: NSJ-RQ5707
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5707-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the C5a receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE mouse HEPA1-6 cells with C5a receptor antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of mouse RAW264.7 cells with C5a receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C5a-R antibody.
3 / 3
Western blot testing of mouse 1) spleen and 2) thymus lysate with C5a receptor antibody. Predicted molecular weight ~43 kDa.
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Immunofluorescent staining of FFPE mouse HEPA1-6 cells with C5a receptor antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of mouse RAW264.7 cells with C5a receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C5a-R antibody.
Western blot testing of mouse 1) spleen and 2) thymus lysate with C5a receptor antibody. Predicted molecular weight ~43 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the C5a receptor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The C5a receptor also known as complement component 5a receptor 1 (C5AR1) or CD88 (Cluster of Differentiation 88) is a G protein-coupled receptor for C5a. It functions as a complement receptor. C5a receptor modulates inflammatory responses, obesity, development and cancers.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids H29-D327) was used as the immunogen for the C5a receptor antibody.
Limitation:
This C5a receptor antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse
Uniprot #:
P30993