AKAP12 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5698
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5698-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AKAP12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE mouse HEPA1-6 cells with AKAP12 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE mouse intestine with AKAP12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of mouse 1) brain and 2) ovary with AKAP12 antibody. Predicted molecular weight: ~191 kDa but can be seen at 250-305 kDa.
4 / 5
Flow cytometry testing of mouse HEPA1-6 cells with AKAP12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKAP12 antibody.
5 / 5
Flow cytometry testing of rat NRK cells with AKAP12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKAP12 antibody.
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Immunofluorescent staining of FFPE mouse HEPA1-6 cells with AKAP12 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse intestine with AKAP12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) brain and 2) ovary with AKAP12 antibody. Predicted molecular weight: ~191 kDa but can be seen at 250-305 kDa.
Flow cytometry testing of mouse HEPA1-6 cells with AKAP12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKAP12 antibody.
Flow cytometry testing of rat NRK cells with AKAP12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKAP12 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AKAP12 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
A-kinase anchor protein 12, also called AKAP250, is an enzyme that in humans is encoded by the AKAP12 gene. The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinase A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids E387-H1620) was used as the immunogen for the AKAP12 antibody.
Limitation:
This AKAP12 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9WTQ5