ADCYAP1 Antibody / PACAP

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NSJ Bioreagents
Product Code: NSJ-F54833
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54833-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54833-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ADCYAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human testis tissue with ADCYAP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining human HEK293 cells with ADCYAP1 antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of human HEK293 cell lysate with ADCYAP1 antibody. Predicted molecular weight ~19 kDa but may be observed at up to 25 kDa.
4 / 4
Flow cytometry testing of human HEK293 cells with ADCYAP1 antibody; Blue=isotype control, Green= ADCYAP1 antibody.
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IHC testing of FFPE human testis tissue with ADCYAP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining human HEK293 cells with ADCYAP1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HEK293 cell lysate with ADCYAP1 antibody. Predicted molecular weight ~19 kDa but may be observed at up to 25 kDa.
Flow cytometry testing of human HEK293 cells with ADCYAP1 antibody; Blue=isotype control, Green= ADCYAP1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ADCYAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ADCYAP1 is adenylate cyclase activating polypeptide 1. Mediated by adenylate cyclase activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene encodes three different mature peptides, including two isotypes, a shorter form and a longer form.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 148-176 from the human protein was used as the immunogen for the ADCYAP1 antibody.
Limitation:
This ADCYAP1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P18509