PIGR Antibody

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NSJ Bioreagents
Product Code: NSJ-F54456
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54456-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54456-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PIGR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human colon tissue with PIGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human hepatocarcinoma tissue with PIGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of human HepG2 cells with PIGR antibody (green) and DAPI nuclear stain (blue).
4 / 5
Western blot testing of human HepG2 cell lysate with PIGR antibody. Predicted molecular weight ~83 kDa.
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Flow cytometry testing of human HepG2 cells with PIGR antibody; Blue=isotype control, Green= PIGR antibody.
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IHC testing of FFPE human colon tissue with PIGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human hepatocarcinoma tissue with PIGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HepG2 cells with PIGR antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HepG2 cell lysate with PIGR antibody. Predicted molecular weight ~83 kDa.
Flow cytometry testing of human HepG2 cells with PIGR antibody; Blue=isotype control, Green= PIGR antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PIGR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PIGR binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 646-672 from the human protein was used as the immunogen for the PIGR antibody.
Limitation:
This PIGR antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P01833