Granulocyte-Macrophage CSF Antibody / CSF2

NSJ Bioreagents
Product Code: NSJ-F54554
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54554-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54554-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CSF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human lung carcinoma tissue with CSF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HEK293 cells with CSF2 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human HL60 cell lysate with CSF2 antibody. Expected molecular weight: 14-35 kDa depending on glycosylation.
4 / 4
Flow cytometry testing of human HL60 cells with CSF2 antibody; Blue=isotype control, Green= CSF2 antibody.

IHC testing of FFPE human lung carcinoma tissue with CSF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK293 cells with CSF2 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HL60 cell lysate with CSF2 antibody. Expected molecular weight: 14-35 kDa depending on glycosylation.
Flow cytometry testing of human HL60 cells with CSF2 antibody; Blue=isotype control, Green= CSF2 antibody.

Further Information

Application Details :
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CSF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CSF2 is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 59-85 from the human protein was used as the immunogen for the CSF2 antibody.
Limitation:
This CSF2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04141