Adrenomedullin antibody / ADM

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NSJ Bioreagents
Product Code: NSJ-F54412
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54412-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54412-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the Adrenomedullin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human MCF7 cell lysate with Adrenomedullin antibody.
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Western blot testing of human 1) breast, 2) kidney and 3) lung lysate with Adrenomedullin antibody.
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Western blot testing of mouse lung lysate with Adrenomedullin antibody.
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Flow cytometry testing of fixed and permeabilized human A549 cells with Adrenomedullin antibody; Blue=isotype control, Green= Adrenomedullin antibody.
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Western blot testing of human MCF7 cell lysate with Adrenomedullin antibody.
Western blot testing of human 1) breast, 2) kidney and 3) lung lysate with Adrenomedullin antibody.
Western blot testing of mouse lung lysate with Adrenomedullin antibody.
Flow cytometry testing of fixed and permeabilized human A549 cells with Adrenomedullin antibody; Blue=isotype control, Green= Adrenomedullin antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Adrenomedullin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ADM, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 69-96 from the human protein was used as the immunogen for the Adrenomedullin antibody.
Limitation:
This Adrenomedullin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P35318