Autotaxin Antibody / ENPP2

NSJ Bioreagents
Product Code: NSJ-F54328
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54328-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54328-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the Autotaxin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human brain lysate with Autotaxin antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
2 / 4
Western blot testing of human 1) placenta and 2) plasma lysate with Autotaxin antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293T cell lysate with Autotaxin antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of human MDA-MB-435 cells with Autotaxin antibody; Blue=isotype control, Green= Autotaxin antibody.

Western blot testing of human brain lysate with Autotaxin antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
Western blot testing of human 1) placenta and 2) plasma lysate with Autotaxin antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
Western blot testing of 1) non-transfected and 2) transfected 293T cell lysate with Autotaxin antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
Flow cytometry testing of human MDA-MB-435 cells with Autotaxin antibody; Blue=isotype control, Green= Autotaxin antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Autotaxin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 377-406 from the human protein was used as the immunogen for the Autotaxin antibody.
Limitation:
This Autotaxin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13822