JAM-A Antibody / Junctional adhesion molecule 1

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NSJ Bioreagents
Product Code: NSJ-RQ5813
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5813-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the F11R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with F11R antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) placenta, 2) Caco-2, 3) MDA-MB-453 and 4) HepG2 lysate with F11R antibody. Expected molecular weight: 35~43kDa depending on glycosylation level.
3 / 3
Flow cytometry testing of human HepG2 cells with F11R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= F11R antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with F11R antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) Caco-2, 3) MDA-MB-453 and 4) HepG2 lysate with F11R antibody. Expected molecular weight: 35~43kDa depending on glycosylation level.
Flow cytometry testing of human HepG2 cells with F11R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= F11R antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the F11R antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Junctional adhesion molecule A is a protein that in humans is encoded by the F11R gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RVEWKFDQGDTTRLVCYNNK from the human protein were used as the immunogen for the F11R antibody.
Limitation:
This F11R antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9Y624