ATG7 Antibody / APG7

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NSJ Bioreagents
Product Code: NSJ-RQ5797
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5797-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ATG7/APG7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-87 MG cells with ATG7/APG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7/APG7 antibody.
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Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) HL-60, 5) Jurkat and 6) ThP-1 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
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Flow cytometry testing of human U-87 MG cells with ATG7/APG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7/APG7 antibody.
Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) HL-60, 5) Jurkat and 6) ThP-1 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ATG7/APG7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D7-Q690) was used as the immunogen for the ATG7/APG7 antibody.
Limitation:
This ATG7/APG7 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O95352