AXL Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6041
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6041-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the AXL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HeLa cells with AXL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AXL antibody.
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Western blot testing of human 1) A431, 2) U-2 OS, 3) U-87 MG, 4) PC-3 and 5) K562 lysate with AXL antibody. Expected molecular weight: 104-140 kDa depending on glycosylation level.
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Flow cytometry testing of human HeLa cells with AXL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AXL antibody.
Western blot testing of human 1) A431, 2) U-2 OS, 3) U-87 MG, 4) PC-3 and 5) K562 lysate with AXL antibody. Expected molecular weight: 104-140 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AXL antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. This gene is mapped to 19q13.2. The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A47-A244) was used as the immunogen for the AXL antibody.
Limitation:
This AXL antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P30530