RGS18 Antibody

NSJ Bioreagents
Product Code: NSJ-RQ5759
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5759-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RGS18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U937 cells with RGS18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RGS18 antibody.
2 / 2
Western blot testing of human K562 lysate with RGS18 antibody. Predicted molecular weight: ~28 kDa.

Flow cytometry testing of human U937 cells with RGS18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RGS18 antibody.
Western blot testing of human K562 lysate with RGS18 antibody. Predicted molecular weight: ~28 kDa.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RGS18 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Regulator of G-protein signaling 18 is a protein that in humans is encoded by the RGS18 gene. This gene encodes a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D230) was used as the immunogen for the RGS18 antibody.
Limitation:
This RGS18 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9NS28