DAI Antibody / ZBP1 / Z-DNA-binding protein 1

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NSJ Bioreagents
Product Code: NSJ-RQ6447
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6447-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the DAI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HeLa cells with DAI antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HEK293, 2) HeLa, 3) K562 and 4) Caco-2 cell lysate with DAI antibody. Predicted molecular weight: 15-49 kDa (multiple isoforms) but can be observed at up to ~60 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with DAI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DAI antibody.
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Immunofluorescent staining of FFPE human HeLa cells with DAI antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293, 2) HeLa, 3) K562 and 4) Caco-2 cell lysate with DAI antibody. Predicted molecular weight: 15-49 kDa (multiple isoforms) but can be observed at up to ~60 kDa.
Flow cytometry testing of human U-87 MG cells with DAI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DAI antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DAI antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Z-DNA-binding protein 1, also known as DNA-dependent activator of IFN-regulatory factors (DAI) and DLM-1, is a protein that in humans is encoded by the ZBP1 gene. This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A2-E421) was used as the immunogen for the DAI antibody.
Limitation:
This DAI antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9H171