Glypican 5 Antibody / GPC5

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NSJ Bioreagents
Product Code: NSJ-RQ5885
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5885-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Glypican 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Flow cytometry testing of human U-87 MG cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
2 / 4
Flow cytometry testing of human U-2 OS cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
3 / 4
Western blot testing of human 1) HeLa, 2) U-87 MG and 3) U-2 OS lysate with Glypican 5 antibody. Predicted molecular weight ~64 kDa.
4 / 4
Immunofluorescent staining of FFPE human A431 cells with Glypican 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human U-87 MG cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
Flow cytometry testing of human U-2 OS cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
Western blot testing of human 1) HeLa, 2) U-87 MG and 3) U-2 OS lysate with Glypican 5 antibody. Predicted molecular weight ~64 kDa.
Immunofluorescent staining of FFPE human A431 cells with Glypican 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Glypican 5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q28-D538) was used as the immunogen for the Glypican 5 antibody.
Limitation:
This Glypican 5 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P78333