IGF2-AS Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6136
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6136-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the IGF2-AS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with IGF2-AS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human PANC-1 cell lysate with IGF2-AS antibody. Predicted molecular weight ~18 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with IGF2-AS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IGF2-AS antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with IGF2-AS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human PANC-1 cell lysate with IGF2-AS antibody. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human Caco-2 cells with IGF2-AS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IGF2-AS antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the IGF2-AS antibody should be determined by the researcher.
Description:
This gene is expressed in antisense to the insulin-like growth factor 2 (IGF2) gene and is imprinted and paternally expressed. It is thought to be non-coding because the putative protein is not conserved and translation is predicted to trigger nonsense mediated decay (NMD). Transcripts from this gene are produced in tumors and may function to suppress cell growth. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-K168) was used as the immunogen for the IGF2-AS antibody.
Limitation:
This IGF2-AS antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q6U949