CDK13 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6249
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6249-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CDK13 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human esophageal squamous carcinoma with CDK13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Immunofluorescent staining of FFPE human HeLa cells with CDK13 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
Western blot testing of human 1) ThP-1, 2) K562 and 3) HL60 cell lysate. Expected molecular weight ~180 kDa.
4 / 4
Flow cytometry testing of human K562 cells with CDK13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK13 antibody.
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IHC staining of FFPE human esophageal squamous carcinoma with CDK13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with CDK13 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) ThP-1, 2) K562 and 3) HL60 cell lysate. Expected molecular weight ~180 kDa.
Flow cytometry testing of human K562 cells with CDK13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK13 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDK13 antibody should be determined by the researcher.
Description:
Cyclin dependent kinase 13 is an enzyme that in humans is encoded by the CDK13 gene. The protein encoded by this gene is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. The exact function of this protein has not yet been determined, but it may play a role in mRNA processing and may be involved in regulation of hematopoiesis. Alternatively spliced transcript variants have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids D1052-N1338) was used as the immunogen for the CDK13 antibody.
Limitation:
This CDK13 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q14004