METTL14 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6199
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6199-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the METTL14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A431 cells with METTL14 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human breast cancer negative control (left) and with METTL14 antibody (right). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) A549, 2) Raji and 3) A431 cell lysate with METTL14 antibody. Expected molecular weight: 52-65 kDa.
4 / 4
Flow cytometry testing of human ThP-1 cells with METTL14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= METTL14 antibody.
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Immunofluorescent staining of FFPE human A431 cells with METTL14 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer negative control (left) and with METTL14 antibody (right). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) Raji and 3) A431 cell lysate with METTL14 antibody. Expected molecular weight: 52-65 kDa.
Flow cytometry testing of human ThP-1 cells with METTL14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= METTL14 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the METTL14 antibody should be determined by the researcher.
Description:
Methyltransferase like 14 is a protein that in humans is encoded by the METTL14 gene. METTL14 is part of a methyltransferase complex required for the common N6-methyladenosine (m6A) base modification that is enriched near stop codons and in long exons of mRNAs.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids Q12-D350) was used as the immunogen for the METTL14 antibody.
Limitation:
This METTL14 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9HCE5