AFM Antibody / Afamin

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NSJ Bioreagents
Product Code: NSJ-RQ5792
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5792-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AFM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human ovarian cancer with Afamin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human liver cancer with Afamin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human plasma lysate with Afamin antibody. Predicted molecular weight: ~69/87 kDa (unmodified/glycosylated).
4 / 4
Flow cytometry testing of human HL-60 cells with Afamin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afamin antibody.
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IHC staining of FFPE human ovarian cancer with Afamin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Afamin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human plasma lysate with Afamin antibody. Predicted molecular weight: ~69/87 kDa (unmodified/glycosylated).
Flow cytometry testing of human HL-60 cells with Afamin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afamin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AFM antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D41-E584) was used as the immunogen for the AFM antibody.
Limitation:
This AFM antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P43652