CHOP Antibody / DDIT3 / GADD153

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NSJ Bioreagents
Product Code: NSJ-RQ5969
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5969-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CHOP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human renal cancer with CHOP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human B lymphocytic tumor tissue with CHOP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human lung cancer with CHOP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Western blot testing of human 1) U-87 MG, 2) K562 and 3) ThP-1 lysate with CHOP antibody. Expected molecular weight: 19-29 kDa.
5 / 6
Flow cytometry testing of human ThP-1 cells with CHOP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDIT3 antibody.
6 / 6
Flow cytometry testing of human K562 cells with CHOP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDIT3 antibody.
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IHC staining of FFPE human renal cancer with CHOP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human B lymphocytic tumor tissue with CHOP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with CHOP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-87 MG, 2) K562 and 3) ThP-1 lysate with CHOP antibody. Expected molecular weight: 19-29 kDa.
Flow cytometry testing of human ThP-1 cells with CHOP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDIT3 antibody.
Flow cytometry testing of human K562 cells with CHOP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDIT3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CHOP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotictranscription factor that is encoded by the DDIT3 gene. It is mapped to 12q13.3. This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-A169) was used as the immunogen for the CHOP antibody.
Limitation:
This CHOP antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P35638