Fibrinogen alpha chain Antibody (FGA)

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NSJ Bioreagents
Product Code: NSJ-RQ6053
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6053-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FGA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human placenta with FGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human SiHa cells with FGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGA antibody.
3 / 4
Flow cytometry testing of human HepG2 cells with FGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGA antibody.
4 / 4
Western blot testing of human 1) T-47D, 2) Caco-2 and 3) SW620 lysate with FGA antibody. Predicted molecular weight ~70 kDa (alpha isoform), ~95 kDa (alpha-E isoform).
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IHC staining of FFPE human placenta with FGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human SiHa cells with FGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGA antibody.
Flow cytometry testing of human HepG2 cells with FGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGA antibody.
Western blot testing of human 1) T-47D, 2) Caco-2 and 3) SW620 lysate with FGA antibody. Predicted molecular weight ~70 kDa (alpha isoform), ~95 kDa (alpha-E isoform).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the FGA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Fibrinogen alpha chain is a protein that in humans is encoded by the FGA gene. This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E139-Q784) was used as the immunogen for the FGA antibody.
Limitation:
This FGA antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P02671