Gasdermin D Antibody / GSDMD

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ5887
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5887-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Gasdermin D antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human rectal cancer with Gasdermin D antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Western blot testing of human 1) SW620 and 2) Jurkat lysate with Gasdermin D antibody. Predicted molecular weight ~53 kDa.
3 / 4
Flow cytometry testing of human U-87 MG cells with Gasdermin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gasdermin D antibody.
4 / 4
Flow cytometry testing of human ThP-1 cells with Gasdermin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gasdermin D antibody.
❮❯

IHC staining of FFPE human rectal cancer with Gasdermin D antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) SW620 and 2) Jurkat lysate with Gasdermin D antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human U-87 MG cells with Gasdermin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gasdermin D antibody.
Flow cytometry testing of human ThP-1 cells with Gasdermin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gasdermin D antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Gasdermin D antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-H484) was used as the immunogen for the Gasdermin D antibody.
Limitation:
This Gasdermin D antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P57764