LY6E Antibody / SCA-2

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NSJ Bioreagents
Product Code: NSJ-RQ6323
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6323-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LY6E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with LY6E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human placental tissue with LY6E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human A549 cell lysate with LY6E antibody. Predicted molecular weight ~14 kDa.
4 / 4
Flow cytometry testing of human SiHa cells with LY6E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LY6E antibody.
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IHC staining of FFPE human ovarian serous adenocarcinoma tissue with LY6E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with LY6E antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate with LY6E antibody. Predicted molecular weight ~14 kDa.
Flow cytometry testing of human SiHa cells with LY6E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LY6E antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LY6E antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Lymphocyte antigen 6E is a protein that in humans is encoded by the LY6E gene. Increased expression of Ly6E is associated with poor survival outcome in multiple malignancies as determined by a survey of more than 130 published clinical studies of gene expression studies on cancer tissue samples and adjacent normal tissues. Ly6E is associated with drug resistance and tumor immune escape in breast cancer. Further research is required to validate Ly6E for translation research.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids L21-S101) was used as the immunogen for the LY6E antibody.
Limitation:
This LY6E antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q16553