Phospholipid transfer protein Antibody / PLTP

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NSJ Bioreagents
Product Code: NSJ-RQ6393
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6393-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Phospholipid transfer protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human ovarian cancer with Phospholipid transfer protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with Phospholipid transfer protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human breast cancer with Phospholipid transfer protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human PC-3 cells with Phospholipid transfer protein antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of human 1) HEK293, 2) SW620, 3) HeLa, 4) PC-3, 5) A549, 6) SGC-7901, 7) Caco-2 and 8) Jurkat cell lysate with Phospholipid transfer protein antibody. Predicted molecular weight ~55 kDa.
6 / 6
Flow cytometry testing of human Caco-2 cells with Phospholipid transfer protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phospholipid transfer protein antibody.
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IHC staining of FFPE human ovarian cancer with Phospholipid transfer protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Phospholipid transfer protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Phospholipid transfer protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human PC-3 cells with Phospholipid transfer protein antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293, 2) SW620, 3) HeLa, 4) PC-3, 5) A549, 6) SGC-7901, 7) Caco-2 and 8) Jurkat cell lysate with Phospholipid transfer protein antibody. Predicted molecular weight ~55 kDa.
Flow cytometry testing of human Caco-2 cells with Phospholipid transfer protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phospholipid transfer protein antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Phospholipid transfer protein antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E18-E470) was used as the immunogen for the Phospholipid transfer protein antibody.
Limitation:
This Phospholipid transfer protein antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P55058