Serum Response Factor Antibody / SRF

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NSJ Bioreagents
Product Code: NSJ-RQ6341
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6341-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Serum Response Factor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE human breast cancer with Serum Response Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain with Serum Response Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain with Serum Response Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
Immunofluorescent staining of FFPE human MCF7 cells with Serum Response Factor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 7
Western blot testing of human 1) HeLa, 2) HEK293, 3) MCF7, 4) Jurkat, 5) ThP-1, 6) Caco-2, 7) rat C6 and 8) mouse NIH 3T3 lysate with Serum Response Factor antibody. Predicted molecular weight: ~52/60-70 kDa (unmodified/phosphorylated).
6 / 7
Flow cytometry testing of human A431 cells with Serum Response Factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Serum Response Factor antibody.
7 / 7
Flow cytometry testing of human U-251 cells with Serum Response Factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Serum Response Factor antibody.
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IHC staining of FFPE human breast cancer with Serum Response Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with Serum Response Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Serum Response Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with Serum Response Factor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HEK293, 3) MCF7, 4) Jurkat, 5) ThP-1, 6) Caco-2, 7) rat C6 and 8) mouse NIH 3T3 lysate with Serum Response Factor antibody. Predicted molecular weight: ~52/60-70 kDa (unmodified/phosphorylated).
Flow cytometry testing of human A431 cells with Serum Response Factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Serum Response Factor antibody.
Flow cytometry testing of human U-251 cells with Serum Response Factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Serum Response Factor antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Serum Response Factor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Serum response factor, also known as SRF, is a transcription factor protein. This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K154-E508) was used as the immunogen for the Serum Response Factor antibody.
Limitation:
This Serum Response Factor antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P11831