MEK5 Antibody / MAP2K5

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NSJ Bioreagents
Product Code: NSJ-RQ6324
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6324-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MEK5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human T-47D cells with MEK5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human skeletal muscle with MEK5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) HEK293, 2) A549, 3) K562, 4) A549, 5) HeLa, 6) PC-3, 7) SW620, 8) monkey COS-7 and 8) rat testis tissue lysate with MEK5 antibody. Predicted molecular weight ~50 kDa.
4 / 4
Flow cytometry testing of human 293T cells with MEK5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MEK5 antibody.
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Immunofluorescent staining of FFPE human T-47D cells with MEK5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human skeletal muscle with MEK5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEK293, 2) A549, 3) K562, 4) A549, 5) HeLa, 6) PC-3, 7) SW620, 8) monkey COS-7 and 8) rat testis tissue lysate with MEK5 antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human 293T cells with MEK5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MEK5 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MEK5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Dual specificity mitogen-activated protein kinase kinase 5 is an enzyme that in humans is encoded by the MAP2K5 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids F9-R442) was used as the immunogen for the MEK5 antibody.
Limitation:
This MEK5 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13163