LSD1 Antibody / Lysine-Specific Demethylase 1

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NSJ Bioreagents
Product Code: NSJ-N1154
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-N1154-25UG25 ug£254.00
Quantity:
NSJ-N1154-100UG100 ug£450.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Aliquot the LSD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human samples with LSD1 antibody at 0.5ug/ml. Expected molecular weight ~110 kDa.
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Western blot testing of mouse samples with LSD1 antibody at 0.5ug/ml. Expected molecular weight ~110 kDa.
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IHC testing of FFPE human testis tissue with LSD1 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 4
IHC testing of FFPE human breast tissue with LSD1 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human samples with LSD1 antibody at 0.5ug/ml. Expected molecular weight ~110 kDa.
Western blot testing of mouse samples with LSD1 antibody at 0.5ug/ml. Expected molecular weight ~110 kDa.
IHC testing of FFPE human testis tissue with LSD1 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human breast tissue with LSD1 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml, IHC (FFPE): 5ug/ml
Application Note:
Provided assay concentrations are suggestions only, LSD1 antibody titration may be required for optimal results.
Buffer:
1X PBS, pH 7.4
Description:
LSD1 (Lysine-specific demethylase 1), also called KDM1 (lysine (K)-specific demethylase 1A), is the first histone demethylase to be identified and regulates transcription via demethylation of lysines 4 and 9 of Histone H3. It negatively regulates pro-inflammatory cytokine expression by interacting with the genes promoter region. It also plays a role in tumor development where overexpression of LSD1 correlates with a poor prognosis. It is upregulated in cancers of the bladder, breast, lung, colon, and GI tract as well as neuroblastoma and cells of mesenchymal origin such as chondrosarcoma, osteosarcoma, rhabdomyosarcoma and Ewing's sarcoma (Bennani-Baiti et al, 2012).
Format :
Purified
Formulation :
0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
23028
Immunogen:
A recombinant protein fragment from the N-terminal region of human LSD1 was used as the immunogen for this antibody.
Limitation:
This LSD1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Species Reactivity :
Human, Mouse