DIO2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54462
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54462-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54462-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DIO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of 1) mouse heart and 2) human skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
2 / 5
Western blot testing of human 1) heart and 2) skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
3 / 5
Western blot testing of human MCF7 cell lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
4 / 5
IHC testing of FFPE mouse brain tissue with DIO2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human MCF7 cells with DIO2 antibody; Blue=isotype control, Green= DIO2 antibody.
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Western blot testing of 1) mouse heart and 2) human skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
Western blot testing of human 1) heart and 2) skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
Western blot testing of human MCF7 cell lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
IHC testing of FFPE mouse brain tissue with DIO2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MCF7 cells with DIO2 antibody; Blue=isotype control, Green= DIO2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the DIO2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 165-191 from the human protein was used as the immunogen for the DIO2 antibody.
Limitation:
This DIO2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q92813