GBA Antibody / Lysosomal acid glucosylceramidase

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NSJ Bioreagents
Product Code: NSJ-F54518
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54518-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54518-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Glucosylceramidase beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skeletal muscle tissue with Glucosylceramidase beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) 293T, 2) HeLa, 3) LNCaP, 4) MCF7 and 5) U-2 OS cell lysate with Glucosylceramidase beta antibody. Predicted molecular weight ~60 kDa.
3 / 4
Western blot testing of mouse lung lysate with Glucosylceramidase beta antibody. Predicted molecular weight ~60 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with Glucosylceramidase beta antibody; Blue=isotype control, Green= Glucosylceramidase beta antibody.
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IHC testing of FFPE human skeletal muscle tissue with Glucosylceramidase beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) 293T, 2) HeLa, 3) LNCaP, 4) MCF7 and 5) U-2 OS cell lysate with Glucosylceramidase beta antibody. Predicted molecular weight ~60 kDa.
Western blot testing of mouse lung lysate with Glucosylceramidase beta antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with Glucosylceramidase beta antibody; Blue=isotype control, Green= Glucosylceramidase beta antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Glucosylceramidase beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GC is a protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 337-365 from the human protein was used as the immunogen for the Glucosylceramidase beta antibody.
Limitation:
This Glucosylceramidase beta antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P04062