STEA2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54829
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54829-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54829-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the STEA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms).
2 / 3
IHC testing of FFPE human prostate carcinoma tissue with STEA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Flow cytometry testing of human HEK293 cells with STEA2 antibody; Blue=isotype control, Green= STEA2 antibody.
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Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms).
IHC testing of FFPE human prostate carcinoma tissue with STEA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with STEA2 antibody; Blue=isotype control, Green= STEA2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the STEA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 229-258 from the human protein was used as the immunogen for the STEA2 antibody.
Limitation:
This STEA2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q8NFT2